CRISPR-Cas: a laboratory manual

By:

Doudna, Jennifer

Contributor(s):

Mali, Prashant

Material type: Text

TextPublication details: New York Cold Spring Harbor Laboratory Press 2016Description: x, 181pISBN:

9781621821311(pbk.)

Subject(s):

DDC classification:

572.8 DOU

Summary: "The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided."

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Item type	Current library	Collection	Call number	Status	Date due	Barcode
Book	Plaksha University Library	Biology	572.8 DOU (Browse shelf(Opens below))	Available		005200

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Previous	572.8 COV Fundamentals of systems biology : from synthetic circuits to whole-cell models	572.8 CRE The physical and chemical basis of molecular biology	572.8 DEK Molecular biology: a genetical approach	572.8 DOU CRISPR-Cas: a laboratory manual	572.8 GRE Molecular cloning: a laboratory manual (3 vol. set.)	572.8 GRE Molecular cloning: a laboratory manual (3 vol. set.)	572.8 GRE Molecular cloning: a laboratory manual (3 vol. set.)	Next

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"The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.

Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided."

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