CRISPR-Cas: (Record no. 10977)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01685nam a22002057a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 241203b |||||||| |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781621821311(pbk.) |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8 |
| Item number | DOU |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Doudna, Jennifer |
| 245 ## - TITLE STATEMENT | |
| Title | CRISPR-Cas: |
| Remainder of title | a laboratory manual |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New York |
| Name of publisher, distributor, etc. | Cold Spring Harbor Laboratory Press |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | x, 181p. |
| 500 ## - GENERAL NOTE | |
| General note | https://www.cshlpress.com/default.tpl?cart=1733214811230405533&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=1074 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | "The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.<br/><br/>Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided." |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Carbohydrates |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Bioanalytics |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mali, Prashant |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Book |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 3865 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 12588 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 12589 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12590 |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | bill no. | bill date | Home library | Current library | Date acquired | Source of acquisition | Cost, normal purchase price | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | Biology | PBD/084/2024-25 | 25/11/2024 | Plaksha University Library | Plaksha University Library | 03/12/2024 | Pandey Books Distributor Pvt. Ltd. | 7490.00 | 572.8 DOU | 005200 | 03/12/2024 | 03/12/2024 | Book |