000			01685nam a22002057a 4500
008			241203b |||||||| |||| 00| 0 eng d
020			_a9781621821311(pbk.)
082			_a572.8 _bDOU
100			_aDoudna, Jennifer _912587
245			_aCRISPR-Cas: _ba laboratory manual
260			_aNew York _bCold Spring Harbor Laboratory Press _c2016
300			_ax, 181p.
500			_ahttps://www.cshlpress.com/default.tpl?cart=1733214811230405533&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=1074
520			_a"The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided."
650			_aCarbohydrates _93865
650			_aDNA _912588
650			_aBioanalytics _912589
700			_aMali, Prashant _912590
942			_cBK
999			_c10977 _d10977