TY  - BOOK
AU  - Doudna, Jennifer 
AU  - Mali, Prashant 
TI  - CRISPR-Cas: : a laboratory manual
SN  - 9781621821311(pbk.)
U1  - 572.8  
PY  - 2016///
CY  - New York			
PB  - Cold Spring Harbor Laboratory Press
KW  - Carbohydrates	
KW  - DNA
KW  - Bioanalytics
N1  - https://www.cshlpress.com/default.tpl?cart=1733214811230405533&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=1074
N2  - "The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.

Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided."
ER  -